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ihc if igsf3  (Boster Bio)


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    Boster Bio ihc if igsf3
    Figure 1 <t>IGSF3</t> expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in
    Ihc If Igsf3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+if+igsf3/Anti-IGSF3+Antibody/pm32355822-294-17-32
    Average 90 stars, based on 1 article reviews
    ihc if igsf3 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway."

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    Journal: Annals of translational medicine

    doi: 10.21037/atm.2020.02.14

    Figure 1 IGSF3 expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in
    Figure Legend Snippet: Figure 1 IGSF3 expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in

    Techniques Used: Expressing, Western Blot

    Figure 2 IGSF3 promotes HCC cell proliferation in vitro. (A) Western blot analysis of IGSF3 in L02 and HCC cells (left panel);
    Figure Legend Snippet: Figure 2 IGSF3 promotes HCC cell proliferation in vitro. (A) Western blot analysis of IGSF3 in L02 and HCC cells (left panel);

    Techniques Used: In Vitro, Western Blot

    Figure 3 IGSF3 affects HCC cell proliferation in vitro. (A,B) Western blot analyses of cyclin A, cyclin E, CDK1, and CDK2 levels in
    Figure Legend Snippet: Figure 3 IGSF3 affects HCC cell proliferation in vitro. (A,B) Western blot analyses of cyclin A, cyclin E, CDK1, and CDK2 levels in

    Techniques Used: In Vitro, Western Blot

    Figure 4 IGSF3 enhances HCC cell migration and invasion in vitro. (A,B) Cell migration capability of HCLLM3 and hepG2 cells
    Figure Legend Snippet: Figure 4 IGSF3 enhances HCC cell migration and invasion in vitro. (A,B) Cell migration capability of HCLLM3 and hepG2 cells

    Techniques Used: Migration, In Vitro

    Figure 5 IGSF3 actives the NF-B signaling pathways. (A,B) Characteristic IF images of NF-κB and IKBα in cell transfected with shCON,
    Figure Legend Snippet: Figure 5 IGSF3 actives the NF-B signaling pathways. (A,B) Characteristic IF images of NF-κB and IKBα in cell transfected with shCON,

    Techniques Used: Protein-Protein interactions, Transfection

    Figure 6 IGSF3 promotes HCC cell proliferation in vivo. (A) Characteristic images of the BALB/c nude mice bearing HCC tumor; (B) the
    Figure Legend Snippet: Figure 6 IGSF3 promotes HCC cell proliferation in vivo. (A) Characteristic images of the BALB/c nude mice bearing HCC tumor; (B) the

    Techniques Used: In Vivo

    Figure 7 IGSF3 promotes HCC cell cycle and NF-B signaling pathways in vivo. (A) Western blot analyses of cyclin A, cyclin E, CDK1,
    Figure Legend Snippet: Figure 7 IGSF3 promotes HCC cell cycle and NF-B signaling pathways in vivo. (A) Western blot analyses of cyclin A, cyclin E, CDK1,

    Techniques Used: Protein-Protein interactions, In Vivo, Western Blot

    Related Articles

    Concentration Assay:

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
    Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..

    Western Blot:

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
    Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..

    Immunohistochemistry:

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
    Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..

    Expressing:

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.
    Article Snippet: .. Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test. ..



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    Ihc If Igsf3, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+if+igsf3/IGSF3+Polyclonal+Antibody/pm32355822-294-17-23
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    ihc if igsf3  (Boster Bio)
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    Boster Bio ihc if igsf3
    Figure 1 <t>IGSF3</t> expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in
    Ihc If Igsf3, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+if+igsf3/Anti-IGSF3+Antibody/pm32355822-294-17-32
    Average 90 stars, based on 1 article reviews
    ihc if igsf3 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1 IGSF3 expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 1 IGSF3 expression profiles in HCC and paired non-tumorous samples. (A) A representative western blotting image of IGSF3 in

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: Expressing, Western Blot

    Figure 2 IGSF3 promotes HCC cell proliferation in vitro. (A) Western blot analysis of IGSF3 in L02 and HCC cells (left panel);

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 2 IGSF3 promotes HCC cell proliferation in vitro. (A) Western blot analysis of IGSF3 in L02 and HCC cells (left panel);

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: In Vitro, Western Blot

    Figure 3 IGSF3 affects HCC cell proliferation in vitro. (A,B) Western blot analyses of cyclin A, cyclin E, CDK1, and CDK2 levels in

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 3 IGSF3 affects HCC cell proliferation in vitro. (A,B) Western blot analyses of cyclin A, cyclin E, CDK1, and CDK2 levels in

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: In Vitro, Western Blot

    Figure 4 IGSF3 enhances HCC cell migration and invasion in vitro. (A,B) Cell migration capability of HCLLM3 and hepG2 cells

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 4 IGSF3 enhances HCC cell migration and invasion in vitro. (A,B) Cell migration capability of HCLLM3 and hepG2 cells

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: Migration, In Vitro

    Figure 5 IGSF3 actives the NF-B signaling pathways. (A,B) Characteristic IF images of NF-κB and IKBα in cell transfected with shCON,

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 5 IGSF3 actives the NF-B signaling pathways. (A,B) Characteristic IF images of NF-κB and IKBα in cell transfected with shCON,

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: Protein-Protein interactions, Transfection

    Figure 6 IGSF3 promotes HCC cell proliferation in vivo. (A) Characteristic images of the BALB/c nude mice bearing HCC tumor; (B) the

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 6 IGSF3 promotes HCC cell proliferation in vivo. (A) Characteristic images of the BALB/c nude mice bearing HCC tumor; (B) the

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: In Vivo

    Figure 7 IGSF3 promotes HCC cell cycle and NF-B signaling pathways in vivo. (A) Western blot analyses of cyclin A, cyclin E, CDK1,

    Journal: Annals of translational medicine

    Article Title: The immunoglobulin superfamily member 3 (IGSF3) promotes hepatocellular carcinoma progression through activation of the NF-κB pathway.

    doi: 10.21037/atm.2020.02.14

    Figure Lengend Snippet: Figure 7 IGSF3 promotes HCC cell cycle and NF-B signaling pathways in vivo. (A) Western blot analyses of cyclin A, cyclin E, CDK1,

    Article Snippet: Table S4 Primary antibodies with indicated concentration for WB, IHC, IF Antibody Dilution Company Cat No. WB IHC IF IGSF3 1:400 1:400 1:50 Bioss Bs-9196R PCNA 1:250 BOSTR BM0104 cyclin A 1:250 BOSTER A00700 cyclin E 1:400 BOSTER A00543-1 CDK1 1:400 BOSTER BM0027 CDK2 1:400 BOSTER PB0562 p-IKBα 1:400 Wanleibio WL03120 IKBα 1:400 Wanleibio WL02495 IKBα 1:200 Beyotime AF1282 p-NF-κB 1:400 Wanleibio WL02169 NF-κB 1:400 Wanleibio WL01273b NF-κB 1:200 Beyotime AF1234 GAPDH 1:800 BOSTER BM3876 Ki-67 1:200 BOSTER PB0065 Lamin B 1:1,000 Bioss bs-1840R β-action 1:800 Bioss bsm-33036M Table S5 Relationship between IGSF3 expression and clinicopathologic features (n=49) Features Relative IGSF3 expression P value Low (n=23) High (n=26) Age 0.2092 ≤50 4 9 >50 19 17 Sex 0.1944 Male 22 21 Female 1 5 AFP (μg/L) 0.5276 ≤400 15 20 >400 8 6 HBV 0.7471 Positive 7 6 Negative 16 20 Tumor diameter (cm) 0.0001 ≤5 19 7 >5 4 19 TNM stage 0.0002 I–II 18 6 III–IV 5 20 Vascular invasion 0.1250 Yes 17 24 No 6 2 Lymph node metastasis 0.0045 Yes 0 8 No 23 18 Significance figures are P values for Fisher’s exact test.

    Techniques: Protein-Protein interactions, In Vivo, Western Blot